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Doubling the resolution of a confocal spinning-disk microscope using image scanning microscopy

Shun Qin, Sebastian Isbaner, Ingo Gregor, Jörg Enderlein

Published: 2020-11-26 DOI: 10.1038/s41596-020-00408-x

Fluorescence Microscopy

Image Scanning Microscopy

Confocal Spinning-Disk Microscope

Structured Illumination

Resolution Doubling

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Fluorescence microscopy has become an indispensable tool for cell biology. Recently, super-resolution methods have been developed to overcome the diffraction limit of light and have shown living cells in unprecedented detail. Often, these methods come at
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ACKNOWLEDGMENTS

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Sheppard, C. J., Mehta, S. B. & Heintzmann, R. Superresolution by image scanning microscopy using pixel reassignment. Opt. Lett. 38, 2889–2892 (2013).
Sheppard, C. J., Castello, M., Tortarolo, G., Vicidomini, G. & Diaspro, A. Image formation in image scanning microscopy, including the case of two-photon excitation. JOSA A 34, 1339–1350 (2017).
Van den Eynde, R. et al. Quantitative comparison of camera technologies for cost-effective super-resolution optical fluctuation imaging (SOFI). J. Phys. Photonics 1, 044001 (2019).
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Nakano, A. Spinning-disk confocal microscopy—a cutting-edge tool for imaging of membrane traffic. Cell Struct. Funct. 27, 349–355 (2002).

关键词

Fluorescence Microscopy

Image Scanning Microscopy

Confocal Spinning-Disk Microscope

Structured Illumination

Resolution Doubling

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