Dissolve lipid mixtures with chloroform in glass vials in moles percent as follows:

• PS liposomes: 99.5% brain PS:0.5% Rhod-PE.
• GC/PS nanotubes: 39.5% Galactosylceramide:60% brian PS:0.5% Cy5-PE.

Evaporate chloroform under a stream of nitrogen gas to produce a lipid film on the glass surface.

Dry the lipid film in a vacuum oven for 1h 0m 0s.

Rehydrate the dried lipid films in liposome buffer at a final concentration of 1mg/mL (~1.2millimolar (mM)).

For PS mixtures, form the liposomes by three freeze (liquid N2)–thaw (37°C water bath) cycles.

For GC/PS mixtures, form the lipid nanotubes by a brief vortexing instead of freeze-thaw cycles.

Remove large aggregates by a brief centrifugation (500x g,0h 0m 0s for 0h 5m 0s) and store in the dark at 4°C to avoid photooxidation.