Microtiter plate assays to assess antibiofilm activity against bacteria

Evan F. Haney, Robert E. W. Hancock, Michael J. Trimble

Published: 2021-04-27 DOI: 10.1038/s41596-021-00515-3

antibiofilm activity

microtiter plate assay

biofilm inhibition

crystal violet

2,3,5-triphenyl tetrazolium chloride

AI 解读

Supplementary information

Supplementary Information

Supplementary Figs. 1–6.

Reporting Summary

Supplementary Data 1

Data for Supplementary Figs. 2, 4 and 5

Efficient and safe single-cell cloning of human pluripotent stem cells using the CEPT cocktail

Profiling native pulmonary basement membrane stiffness using atomic force microscopy

Genetic circuit design automation with Cello 2.0

Synthesis of siRNA nanoparticles to silence plaque-destabilizing gene in atherosclerotic lesional macrophages

Scalable dual-omics profiling with single-nucleus chromatin accessibility and mRNA expression sequencing 2 (SNARE-seq2)

A practical guide to scanning light-field microscopy with digital adaptive optics

Effective high-throughput isolation of fully human antibodies targeting infectious pathogens

Improved sampling and DNA extraction procedures for microbiome analysis in food-processing environments

MOF-derived nanoporous carbons with diverse tunable nanoarchitectures

Tutorial: a statistical genetics guide to identifying HLA alleles driving complex disease

查看全部

Sections

Figures

References

Supplementary information

Flemming, H.-C. et al. Biofilms: an emergent form of bacterial life. Nat. Rev. Microbiol. 14, 563–575 (2016).
Fernández, L., Breidenstein, E. B. M. & Hancock, R. E. W. Creeping baselines and adaptive resistance to antibiotics. Drug Resist. Updat. 14, 1–21 (2011).
Bryers, J. D. Medical biofilms. Biotechnol. Bioeng. 100, 1–18 (2008).
Bjarnsholt, T. The role of bacterial biofilms in chronic infections. APMIS 121, 1–58 (2013).
Verderosa, A. D., Totsika, M. & Fairfull-Smith, K. E. Bacterial biofilm eradication agents: a current review. Front. Chem. 7, 824 (2019).
Coenye, T., Kjellerup, B., Stoodley, P. & Bjarnsholt, T. The future of biofilm research—report on the ‘2019 Biofilm Bash’. Biofilm 2, 100012 (2020).
Römling, U. & Balsalobre, C. Biofilm infections, their resilience to therapy and innovative treatment strategies. J. Intern. Med. 272, 541–561 (2012).
Taff, H. T., Nett, J. E. & Andes, D. R. Comparative analysis of Candida biofilm quantitation assays. Med. Mycol. 50, 214–218 (2012).
Haney, E., Trimble, M., Cheng, J., Vallé, Q. & Hancock, R. Critical assessment of methods to quantify biofilm growth and evaluate antibiofilm activity of host defence peptides. Biomolecules 8, 29 (2018).
Wiegand, I., Hilpert, K. & Hancock, R. E. W. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat. Protoc. 3, 163–175 (2008).
Beveridge, T. Use of the Gram stain in microbiology. Biotech. Histochem. 76, 111–118 (2001).
Berridge, M. V., Herst, P. M. & Tan, A. S. Tetrazolium dyes as tools in cell biology: New insights into their cellular reduction. Biotechnol. Annu. Rev. 11, 127–152 (2005).
Sabaeifard, P., Abdi-Ali, A., Soudi, M. R. & Dinarvand, R. Optimization of tetrazolium salt assay for Pseudomonas aeruginosa biofilm using microtiter plate method. J. Microbiol. Methods 105, 134–140 (2014).
Azeredo, J. et al. Critical review on biofilm methods. Crit. Rev. Microbiol. 43, 313–351 (2017).
Peeters, E., Nelis, H. J. & Coenye, T. Comparison of multiple methods for quantification of microbial biofilms grown in microtiter plates. J. Microbiol. Methods 72, 157–165 (2008).
Haney, E. F. et al. Computer-aided discovery of peptides that specifically attack bacterial biofilms. Sci. Rep. 8, 1871 (2018).
Haney, E. F., Mansour, S., Hilchie, A. L., de la Fuente-Núñez, C. & Hancock, R. E. W. High throughput screening methods for assessing antibiofilm and immunomodulatory activities of synthetic peptides. Peptides 71, 276–285 (2015).
de la Fuente-Núñez, C. et al. Inhibition of bacterial biofilm formation and swarming motility by a small synthetic cationic peptide. Antimicrob. Agents Chemother. 56, 2696–2704 (2012).
de la Fuente-Núñez, C., Reffuveille, F., Haney, E. F., Straus, S. K. & Hancock, R. E. W. Broad-spectrum anti-biofilm peptide that targets a cellular stress response. PLoS Pathog. 10, (2014).
Haney, E. F., Barbosa, S. C., Baquir, B. & Hancock, R. E. W. Influence of non-natural cationic amino acids on the biological activity profile of innate defense regulator peptides. J. Med. Chem. 62, 10294–10304 (2019).
Ommen, P., Zobek, N. & Meyer, R. L. Quantification of biofilm biomass by staining: Non-toxic safranin can replace the popular crystal violet. J. Microbiol. Methods 141, 87–89 (2017).
Toté, K., Berghe, D. V., Maes, L. & Cos, P. A new colorimetric microtitre model for the detection of Staphylococcus aureus biofilms. Lett. Appl. Microbiol. 46, 249–254 (2008).
Rajamani, S. et al. Robust biofilm assay for quantification and high throughput screening applications. J. Microbiol. Methods 159, 179–185 (2019).
Navarro, G. et al. Image-based 384-well high-throughput screening method for the discovery of skyllamycins A to C as biofilm inhibitors and inducers of biofilm detachment in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 58, 1092–1099 (2014).
Tremblay, Y. D. N., Vogeleer, P., Jacques, M. & Harel, J. High-throughput microfluidic method to study biofilm formation and host-pathogen interactions in pathogenic Escherichia coli. Appl. Environ. Microbiol. 81, 2827–2840 (2015).
Bridier, A., Dubois-Brissonnet, F., Boubetra, A., Thomas, V. & Briandet, R. The biofilm architecture of sixty opportunistic pathogens deciphered using a high throughput CLSM method. J. Microbiol. Methods 82, 64–70 (2010).
Bernardi, T. et al. High-throughput screening of metal-N-heterocyclic carbene complexes against biofilm formation by pathogenic bacteria. ChemMedChem 9, 1140–1144 (2014).
Olivares, E. et al. The BioFilm Ring Test: a rapid method for routine analysis of Pseudomonas aeruginosa biofilm formation kinetics. J. Clin. Microbiol. 54, 657–661 (2016).
Ceri, H. et al. The Calgary Biofilm Device: new technology for rapid determination of antibiotic susceptibilities of bacterial biofilms. J. Clin. Microbiol. 37, 1771–1776 (1999).
Harrison, J. J. et al. Microtiter susceptibility testing of microbes growing on peg lids: a miniaturized biofilm model for high-throughput screening. Nat. Protoc. 5, 1236–1254 (2010).
Kragh, K. N., Alhede, M., Kvich, L. & Bjarnsholt, T. Into the well—a close look at the complex structures of a microtiter biofilm and the crystal violet assay. Biofilm 1, 100006 (2019).
Trunk, T., Khalil, H. S. & Leo, J. C. Bacterial autoaggregation. AIMS Microbiol 4, 140–164 (2018).
Kragh, K. N. et al. Role of multicellular aggregates in biofilm formation. mBio 7, (2016).
Harrison, J. J. et al. The use of microscopy and three-dimensional visualization to evaluate the structure of microbial biofilms cultivated in the Calgary Biofilm Device. Biol. Proced. Online 8, 194–215 (2006).
Molina-Manso, D. et al. In vitro susceptibility to antibiotics of staphylococci in biofilms isolated from orthopaedic infections. Int. J. Antimicrob. Agents 41, 521–523 (2013).
Ali, L., Khambaty, F. & Diachenko, G. Investigating the suitability of the Calgary Biofilm Device for assessing the antimicrobial efficacy of new agents. Bioresour. Technol. 97, 1887–1893 (2006).
Wong, H. S., Townsend, K. M., Fenwick, S. G., Trengove, R. D. & O’Handley, R. M. Comparative susceptibility of planktonic and 3-day-old Salmonella Typhimurium biofilms to disinfectants. J. Appl. Microbiol. 108, 2222–2228 (2010).
Rivardo, F., Martinotti, M. G., Turner, R. J. & Ceri, H. The activity of silver against Escherichia coli biofilm is increased by a lipopeptide biosurfactant. Can. J. Microbiol. 56, 272–278 (2010).
Karygianni, L., Ren, Z., Koo, H. & Thurnheer, T. Biofilm matrixome: extracellular components in structured microbial communities. Trends Microbiol 28, 668–681 (2020).
Karunakaran, E., Mukherjee, J., Ramalingam, B. & Biggs, C. A. “Biofilmology”: a multidisciplinary review of the study of microbial biofilms. Appl. Microbiol. Biotechnol. 90, 1869–1881 (2011).
Benov, L. Effect of growth media on the MTT colorimetric assay in bacteria. PLoS ONE 14, e0219713 (2019).
Colquhoun, J. M., Wozniak, R. A. F. & Dunman, P. M. Clinically relevant growth conditions alter Acinetobacter baumannii antibiotic susceptibility and promote identification of novel antibacterial agents. PLoS ONE 10, e0143033 (2015).
Seneviratne, C. J., Yip, J. W. Y., Chang, J. W. W., Zhang, C. F. & Samaranayake, L. P. Effect of culture media and nutrients on biofilm growth kinetics of laboratory and clinical strains of Enterococcus faecalis. Arch. Oral Biol. 58, 1327–1334 (2013).
Palmer, K. L., Aye, L. M. & Whiteley, M. Nutritional cues control Pseudomonas aeruginosa multicellular behavior in cystic fibrosis sputum. J. Bacteriol. 189, 8079–8087 (2007).
Pompilio, A. et al. Potential novel therapeutic strategies in cystic fibrosis: antimicrobial and anti-biofilm activity of natural and designed α-helical peptides against Staphylococcus aureus, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia. BMC Microbiol 12, 145 (2012).
Haney, E. F. et al. Aggregation and its influence on the immunomodulatory activity of synthetic innate defense regulator peptides. Cell Chem. Biol. 24, 969–980.e4 (2017).
O’Toole, G. A. Microtiter dish biofilm formation assay. J. Vis. Exp. http://doi.org/10.3791/2437 (2011).
Coffey, B. M. & Anderson, G. G. in Pseudomonas Methods and Protocols (eds Filloux, A. & Ramos, J.-L.) 631–641 (Springer, 2014).
de la Fuente-Núñez, C. et al. D-enantiomeric peptides that eradicate wild-type and multidrug-resistant biofilms and protect against lethal Pseudomonas aeruginosa infections. Chem. Biol. 22, 196–205 (2015).
McBain, A. J. in Advances in Applied Microbiology Vol. 69, Ch. 4, 99–132 (Academic Press, 2009).
Lebeaux, D., Chauhan, A., Rendueles, O. & Beloin, C. From in vitro to in vivo models of bacterial biofilm-related infections. Pathogens 2, 288–356 (2013).

Microtiter plate assays to assess antibiofilm activity against bacteria

Supplementary information

Supplementary Information

Reporting Summary

Supplementary Data 1

推荐阅读