Screening circular RNAs with functional potential using the RfxCas13d/BSJ-gRNA system

Hao Wu, Ling-Ling Chen, Siqi Li

Published: 2022-07-12 DOI: 10.1038/s41596-022-00715-5

circRNA screening

RfxCas13d

BSJ-gRNA

functional annotation

CRISPR–Cas13

AI 解读

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Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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References

Circular RNAs (circRNAs) are covalently enclosed, single-stranded RNAs produced by back-splicing of pre-mRNA exons that have recently emerged as an important class of molecules in gene expression regulation. circRNAs share overlapping sequences with their
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
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References
ACKNOWLEDGMENTS

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Screening circular RNAs with functional potential using the RfxCas13d/BSJ-gRNA system

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