Yeast-based bioproduction of disulfide-rich peptides and their cyclization via asparaginyl endopeptidases

Kuok Yap, Junqiao Du, Fabian B. H. Rehm, Shyn Ric Tang, Yan Zhou, Jing Xie, Conan K. Wang, Simon J. de Veer, Linda H. L. Lua, Thomas Durek, David J. Craik

Published: 2021-02-16 DOI: 10.1038/s41596-020-00483-0

Pichia pastoris

cyclic peptides

asparaginyl endopeptidases

bioproduction

enzymatic cyclization

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Supplementary information

Supplementary Information

Supplementary Figs. 1–3.

Reporting Summary

Generation of mouse–human chimeric embryos

Genome-wide quantification of transcription factor binding at single-DNA-molecule resolution using methyl-transferase footprinting

Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

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Decoding personal biotic and abiotic airborne exposome

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Single-cell RNA sequencing (scRNA-seq)（单细胞RNA测序）

Data integration（数据集成）

Heterogeneity（异质性）

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References

Supplementary information

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Yeast-based bioproduction of disulfide-rich peptides and their cyclization via asparaginyl endopeptidases

Supplementary information

Supplementary Information

Reporting Summary

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